Instructors: Guo-Liang Wang and Eric Stocki

Click below to download handouts and reference papers as PDF files

One slides/page lecture notes

Four slides/page lecture notes

Reference paper 1

Reference paper 2

Course Video

Printed copies of handouts will not be provided in class. Please print out your own handouts.

Study questions

1. What are the two classes of transposable elements? Describe their mechanism of transposition and use for insertion mutagenesis. Compare the advantages and the disadvantages of Ac/Ds and Tos17 tagging systems.
2. Describe the procedure of T-DNA mutant generation in Arabidopsis. Describe the advantages and the disadvantages of the T-DNA tagging system for the generation of mutant populations. What is the advantage of activation T-DNA tagging over the traditional T-DNA tagging? How does it work?
3. Describe three methods you can use to identify the flanking sequence of the inserted transposon or T-DNA. List the steps you will take to prove whether the isolated candidate gene is the one responsible for the mutant phenotype.
4. List two methods to isolate the mutated genes from deletion mutants and naturally occurring germplasm collections. Describe the procedure of the map-based cloning strategy.
5. What are the problems/difficulties of traditional map-based cloning? Describe the principle of microarray-based mutation detection and gene cloning strategy.