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Lecture 1

Lecture 2

Lecture 3

Lecture 4

Lecture 5

Lecture 6

Lecture 7

Lecture 8

REVIEW 1

Lecture 9

Lecture 10

Lecture 11

Lecture 12

Lecture 13

Lecture 14

Lecture 15

Lecture 16

Lecture 17

  


PP703 - Agricultural Genomics: Principles and Applications

Instructors: Guo-Liang Wang and Eric Stockinger

 

Click below to download handouts and reference papers as PDF files

One slides/page lecture notes

Four slides/page lecture notes

Reference paper 1

Reference paper 2

Course Video


Printed copies of handouts will not be provided in class. Please print out your own handouts.

Study questions

1. List two methods you can use to isolate the mutant of your genes from insertion mutant populations.
2.What should you do after identifying an insertion mutant of your target genes?
3. What is in silico mutant screen from insertion mutant collections?
4. List two methods you can use to isolate the mutant of your genes from deletion mutant collections. What are the principle and procedure of the TILLING method?
5. List five different methods for targeted gene mutagenesis. What are the problems in sense and anti-sense gene silencing for the generation of mutants? What are the advantages of VIGS over other methods?
6. Briefly describe the mechanism of RNA interference (RNAi)-mediated gene silencing method. What are the advantages and the disadvantages of RNAi mediated gene silencing over others method.
7. Describe the principle of artificial microRNA (amiRNA) method for gene silencing. What are the advantages and disadvantages of amiRNAs approach for gene silencing?